L-Arginine synthesized through fermentation using Escherichia coli strain CCTCC M 20231961
In a recent development, the European Union (EU) has recommended specific methods for determining the essential amino acid L-arginine in animal feed additives, premixtures, compound feed, and water. These methods are essential for ensuring the accuracy and reliability of L-arginine content in various feed formulations.
The European Reference Laboratory (EURL) has endorsed the "L-arginine monograph" of the Food Chemical Codex (FCC) for the identification of L-arginine in feed additives for official control. For the determination of arginine in feed additives, premixtures, and water, the EURL recommends the EN ISO 17180 method based on IEC-VIS/FLD, and for compound feed, the EU method based on IEC-VIS is proposed.
The EU method has been demonstrated to be applicable for the determination of arginine in water, as shown by experimental data provided in the frame of stability studies. Interestingly, no method was proposed by the Applicant for the determination of arginine in water, yet the applicability of the EU method was still demonstrated through these studies.
The Applicant has proposed the ring-trial validated method EN ISO 17180 for the determination of arginine in the feed additive and premixtures. This method involves ion-exchange chromatography (IEC) coupled with post-column derivatisation and optical (VIS) detection.
The performance characteristics of the EU method for the determination of arginine in animal feedingstuffs show a relative standard deviation for repeatability (RSD) ranging from 2.3 to 3.3% and a relative standard deviation for reproducibility (RSD) ranging from 7.2 to 9.7%.
The proposed L-arginine feed additive has a minimum content of 98.5% (w/w, based on dry matter) for use in all animal species. The authorization for this feed additive is sought for all animal species, and it is intended to be added to feed through premixtures or directly into compound feed and water.
These recommendations are in line with Regulation (EC) No 1831/2003, which focuses on the authorization of feed additives and their safety evaluations, and Commission Regulation (EC) No 152/2009, which lays down the methods of sampling and analysis for the official control of feed. Laboratories often follow AOAC International or ISO/IEC 17025 standards for accurate determination of L-arginine, as these standards provide guidelines for analytical methods in the food and feed industries.
For precise guidance on the methods recommended by these regulations, it would be advisable to consult the official documents or contact relevant regulatory bodies in the European Union.
- In the realm of health-and-wellness and nutrition, the European Union (EU) suggests consulting the official documents or contacting relevant regulatory bodies for precise guidance on the methods recommended by Regulation (EC) No 1831/2003 and Commission Regulation (EC) No 152/2009, as these regulations provide guidelines for the accurate determination of L-arginine in various food and feed industries.
- The European Reference Laboratory (EURL) encourages the use of specific methods for determining L-arginine in water, such as the EU method based on IEC-VIS, as demonstrated by experimental data from stability studies, to ensure the data's accuracy and reliability in the field of food science and health-and-wellness.
